Publications – PlewkaLAB – We Crystallize Solutions

Characterization of Clinically Evaluated Small-Molecule Inhibitors of PD-L1 for Immunotherapy

Authors: Alicja Słota; Katarzyna Gołębiowska-Mendroch; Justyna Kocik-Król; Bogdan Musielak; Małgorzata Stec; Kazimierz Węglarczyk; Maciej Paweł Siedlar; Łukasz Skałniak; Jacek Plewka; Katarzyna Magiera-Mularz

DOI: 10.1021/acsmedchemlett.5c00245

Year of publication: 2025

Link: https://pubs.acs.org/doi/10.1021/acsmedchemlett.5c00245

Structural and Functional Characteristics of Potent Dioxygenase from Moesziomyces aphidis

Authors: Lukas Schober; Jacek Plewka; Kanokkan Sriwaiyaphram; Björn Bielec; Astrid Schiefer; Thanyaporn Wongnate; Katarzyna Magiera-Mularz; Florian Rudroff; Margit Winkler

DOI: 10.1021/jacsau.5c00456

Year of publication: 2025

Link: https://pubs.acs.org/doi/10.1021/jacsau.5c00456

1,5-Disubstituted tetrazoles as PD-1/PD-L1 antagonists

Authors: Robin van der Straat; Rosalie Draijer; Ewa Surmiak; Roberto Butera; Lennart Land; Katarzyna Magiera-Mularz; Bogdan Musielak; Jacek Plewka; Tad A. Holak; Alexander Dömling

DOI: 10.1039/D3MD00746D

Year of publication: 2024

Link: https://pubs.rsc.org/en/content/articlelanding/2024/md/d3md00746d

Antibody-Ligand Interactions on a High-Capacity staphylococcal protein A resin

Authors: Rupert Tscheließnig; Gonçalo L. Silva; Jacek Plewka; Leo A. Jakob; Helga C. Lichtenegger; Alois Jungbauer; Ana C. Dias-Cabral

DOI: 10.1016/j.chroma.2024.465102

Year of publication: 2024

Link: https://pubmed.ncbi.nlm.nih.gov/38941799/

Autoinhibition of suicidal capsid protease from O’nyong’nyong virus

Authors: Yuliya Chykunova; Jacek Plewka; Piotr Wilk; Karolina Torzyk; Marcin Sienczyk; Grzegorz Dubin; Krzysztof Pyrc

DOI: 10.1016/j.ijbiomac.2024.130136

Year of publication: 2024

Link: https://pubmed.ncbi.nlm.nih.gov/38354926/

Design, synthesis, and evaluation of antitumor activity of 2-arylmethoxy-4-(2-fluoromethyl-biphenyl-3-ylmethoxy) benzylamine derivatives as PD-1/PD-l1 inhibitors

Authors: Feng Zhang; Hua Zhang; Shijia Zhou; Jacek Plewka; Ming Wang; Shishi Sun; Caiyun Wu; Qimeng Yu; Mengyu Zhu; Annoor Awadasseid; Yanling Wu; Katarzyna Magiera-Mularz; Wen Zhang

DOI: 10.1016/j.ejmech.2024.116683

Year of publication: 2024

Link: https://pubmed.ncbi.nlm.nih.gov/39032403/

Despite the odds: formation of the SARS-CoV-2 methylation complex

Authors: Alex Matsuda, Jacek Plewka, Michał Rawski, André Mourão, Weronika Zajko, Till Siebenmorgen, Leanid Kresik, Kinga Lis, Alisha N Jones, Magdalena Pachota, Abdulkarim Karim, Kinga Hartman, Shivlee Nirwal, Ravi Sonani, Yuliya Chykunova, Igor Minia, Paweł Mak, Markus Landthaler, Marcin Nowotny, Grzegorz Dubin, Michael Sattler, Piotr Suder, Grzegorz M Popowicz, Krzysztof Pyrć, Anna Czarna

DOI: 10.1093/nar/gkae165

Year of publication: 2024

Link: https://academic.oup.com/nar/article/52/11/6441/7631398

Nonsymmetrically Substituted 1,1′-Biphenyl-Based Small Molecule Inhibitors of the PD-1/PD-L1 Interaction

Authors: Aleksandra Hec-Gałązka; Urszula Tyrcha; Jan Barczyński; Przemysław Bielski; Michał Mikitiuk; Ganna P. Gudz; Radosław Kitel; Bogdan Musielak; Jacek Plewka; Tomasz Sitar; Tad A. Holak

DOI: 10.1021/acsmedchemlett.4c00042

Year of publication: 2024

Link: https://pubs.acs.org/doi/10.1021/acsmedchemlett.4c00042

SARS-CoV-2 Mpro oligomerization as a potential target for therapy

Authors: Kinga Lis; Jacek Plewka; Filipe Menezes; Ewa Bielecka; Yuliya Chykunova; Katarzyna Pustelny; Stephan Niebling; Angelica Struve Garcia; Maria Garcia-Alai; Grzegorz M Popowicz; Anna Czarna; Tomasz Kantyka; Krzysztof Pyrc

DOI: 10.1016/j.ijbiomac.2024.131392

Year of publication: 2024

Link: https://pubmed.ncbi.nlm.nih.gov/38582483/

Structural dynamics of the TPR domain of the peroxisomal cargo receptor Pex5 in Trypanosoma

Authors: Michal Banasik, Valeria Napolitano, Artur Blat, Karim Abdulkarim, Jacek Plewka, Cezary Czaplewski, Artur Giełdoń, Maciej Kozak, Benedykt Władyka, Grzegorz Popowicz, Grzegorz Dubin

DOI: 10.1016/j.ijbiomac.2024.135510

Year of publication: 2024

Link: https://pubmed.ncbi.nlm.nih.gov/39304044/

Anti-SARS-CoV-2 activity of cyanopeptolins produced by Nostoc edaphicum CCNP1411

Authors: Robert Konkel, Aleksandra Milewska, Nguyen Dan Thuc Do, Emilia Barreto Duran, Artur Szczepanski, Jacek Plewka, Ewa Wieczerzak, Sofia Iliakopoulou, Triantafyllos Kaloudis, Dirk Jochmans, Johan Neyts, Krzysztof Pyrc, Hanna Mazur-Marzec

DOI: 10.1016/j.antiviral.2023.105731

Year of publication: 2023

Link: https://pubmed.ncbi.nlm.nih.gov/37838220/

Design, Synthesis, and Antitumor Activity Evaluation of 2-Arylmethoxy-4-(2,2′-dihalogen-substituted biphenyl-3-ylmethoxy) Benzylamine Derivatives as Potent PD-1/PD-L1 Inhibitors

Authors: Hua Zhang; Shijia Zhou; Jacek Plewka; Caiyun Wu; Mengyu Zhu; Qimeng Yu; Bogdan Musielak; Xiao Wang; Annoor Awadasseid; Katarzyna Magiera-Mularz; Yanling Wu; Wen Zhang

DOI: 10.1021/acs.jmedchem.3c00731

Year of publication: 2023

Link: https://pubs.acs.org/doi/abs/10.1021/acs.jmedchem.3c00731

Design, Synthesis, and Biological Evaluation of 2-Hydroxy-4-phenylthiophene-3-carbonitrile as PD-L1 Antagonist and Its Comparison to Available Small Molecular PD-L1 Inhibitors

Authors: Marta A. Ważyńska; Roberto Butera; Marta Requesens; Annechien Plat; Tryfon Zarganes-Tzitzikas; Constantinos G. Neochoritis; Jacek Plewka; Lukasz Skalniak; Justyna Kocik-Krol; Bogdan Musielak; Katarzyna Magiera-Mularz; Ismael Rodriguez; Simon N. Blok; Marco de Bruyn; Hans W. Nijman; Philip H. Elsinga; Tad A. Holak; Alexander Dömling

DOI: 10.1021/acs.jmedchem.3c00254

Year of publication: 2023

Link: https://pubs.acs.org/doi/10.1021/acs.jmedchem.3c00254

Monocyte subpopulations display disease-specific miRNA signatures depending on the subform of Spondyloarthropathy

DOI: 10.3389/fimmu.2023.1124894

Year of publication: 2023

Link: https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2023.1124894/full

Solubilizer Tag Effect on PD-L1/Inhibitor Binding Properties for m-Terphenyl Derivatives

Authors: Ewa Surmiak; Julia Ząber; Jacek Plewka; Grzegorz Wojtanowicz; Justyna Kocik-Król; Oskar Kruc; Damian Muszak; Ismael Rodríguez; Bogdan Musielak; Monica Viviano; Sabrina Castellano; Łukasz Skalniak; Katarzyna Magiera-Mularz; Tad A. Holak; Justyna Kalinowska-Tłuścik

DOI: 10.1021/acsmedchemlett.3c00306

Year of publication: 2023

Link: https://pubs.acs.org/doi/10.1021/acsmedchemlett.3c00306

Structural and biological characterization of pAC65, a macrocyclic peptide that blocks PD-L1 with equivalent potency to the FDA-approved antibodies

Authors: Ismael Rodríguez; Justyna Kocik-Król; Łukasz Skałniak; Bogdan Musielak; Aneta Wiśniewska; Agnieszka Ciesiołkiewicz; Lukasz Berlicki; Jacek Plewka; Przemysław Grudnik; Małgorzata Stec; Maciej Siedlar; Tad A. Holak; Katarzyna Magiera-Mularz

DOI: 10.1186/s12943-023-01853-4

Year of publication: 2023

Link: https://molecular-cancer.biomedcentral.com/articles/10.1186/s12943-023-01853-4

Novel IL2RG Gene Mutation in One of Dizygotic Twins Causing Profound Changes of Receptor Structure

Authors: Magdalena Rutkowska-Zapała; Anna Szaflarska; Anna Kluczewska; Julia Cięciwa; Jacek Plewka; Anna Michalska; Maciej Siedlar

DOI: 10.3389/fped.2022.858166

Year of publication: 2022

Link: https://www.frontiersin.org/journals/pediatrics/articles/10.3389/fped.2022.858166/full

Pseudanabaena galeata CCNP1313—Biological Activity and Peptides Production

Authors: Marta Cegłowska; Karolina Szubert; Beata Grygier; Marzena Lenart; Jacek Plewka; Aleksandra Milewska; Kinga Lis; Artur Szczepański; Yuliya Chykunova; Emilia Barreto-Duran; Krzysztof Pyrć; Alicja Kosakowska; Hanna Mazur-Marzec

DOI: 10.3390/toxins14050330

Year of publication: 2022

Link: https://www.mdpi.com/2072-6651/14/5/330

Refolding of lid subdomain of SARS-CoV-2 nsp14 upon nsp10 interaction releases exonuclease activity

Authors: Anna Czarna; Jacek Plewka; Leanid Kresik; Alex Matsuda; Abdulkarim Karim; Colin Robinson; Sean O’Byrne; Fraser Cunningham; Irene Georgiou; Piotr Wilk; Magdalena Pachota; Grzegorz Popowicz; Paul Graham Wyatt; Grzegorz Dubin; Krzysztof Pyrć

DOI: 10.1016/j.str.2022.04.014

Year of publication: 2022

Link: https://www.cell.com/structure/fulltext/S0969-2126(22)00174-5?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS0969212622001745%3Fshowall%3Dtrue

A computationally designed β-amino acid-containing miniprotein

Authors: Magdalena Bejger; Paulina Fortuna; Magda Drewniak-Świtalska; Jacek Plewka; Wojciech Rypniewski; Łukasz Berlicki

DOI: 10.1039/D1CC02192C

Year of publication: 2021

Link: https://pubs.rsc.org/en/content/articlelanding/2021/cc/d1cc02192c

A fragment-based approach identifies an allosteric pocket that impacts malate dehydrogenase activity

Authors: Atilio Reyes Romero, Serjey Lunev, Grzegorz M. Popowicz, Vito Calderone, Matteo Gentili, Michael Sattler, Jacek Plewka, Michał Taube, Maciej Kozak, Tad A. Holak, Alexander S. S. Dömling, Matthew R. Groves

DOI: 10.1038/s42003-021-02442-1

Year of publication: 2021

Link: https://www.nature.com/articles/s42003-021-02442-1

Design, Synthesis, and Biological Evaluation of Imidazopyridines as PD-1/PD-L1 Antagonists

Authors: Roberto Butera; Marta Ważyńska; Katarzyna Magiera-Mularz; Jacek Plewka; Bogdan Musielak; Ewa Surmiak; Dominik Sala; Radoslaw Kitel; Marco de Bruyn; Hans W. Nijman; Philip H. Elsinga; Tad A. Holak; Alexander Dömling

DOI: 10.1021/acsmedchemlett.1c00033

Year of publication: 2021

Link: https://pubs.acs.org/doi/10.1021/acsmedchemlett.1c00033

PD-L1 Inhibitors: Different Classes, Activities, and Mechanisms of Action

Authors: Ewa Surmiak; Katarzyna Magiera-Mularz; Bogdan Musielak; Damian Muszak; Justyna Kocik-Krol; Radoslaw Kitel; Jacek Plewka; Tad A. Holak; Lukasz Skalniak

DOI: 10.3390/ijms222111797

Year of publication: 2021

Link: https://www.mdpi.com/1422-0067/22/21/11797

Terphenyl-Based Small-Molecule Inhibitors of Programmed Cell Death-1/Programmed Death-Ligand 1 (PD-1/PD-L1) Protein–Protein Interaction

Authors: Damian Muszak; Ewa Surmiak; Jacek Plewka; Katarzyna Magiera-Mularz; Justyna Kocik-Król; Bogdan Musielak; Dominik Sala; Radosław Kitel; Małgorzata Stec; Kazimierz Węglarczyk; Maciej Siedlar; Alexander Dömling; Łukasz Skalniak; Tad A. Holak

DOI: 10.1021/acs.jmedchem.1c00957

Year of publication: 2021

Link: https://pubs.acs.org/doi/10.1021/acs.jmedchem.1c00957

A bacteriophage mimic of the bacterial nucleoid-associated protein Fis

Authors: Soumyananda Chakraborti; Dhanasekaran Balakrishnan; Alexander J. Trotter; William H. Gittens; Ally W.H. Yang; Arttu Jolma; Joy R. Paterson; Sylwia Świątek; Jacek Plewka; Fiona A. Curtis; Laura Y. Bowers; Lars-Olof Pålsson; Timothy R. Hughes; Michał Taube; Maciej Kozak; Jonathan G. Heddle; Gary J. Sharples

DOI: 10.1042/BCJ20200146

Year of publication: 2020

Link: https://portlandpress.com/biochemj/article/477/7/1345/222455/A-bacteriophage-mimic-of-the-bacterial-nucleoid

Competition NMR for Detection of Hit/Lead Inhibitors of Protein–Protein Interactions

Authors: Bogdan Musielak; Weronika Janczyk; Ismael Rodriquez; Jacek Plewka; Dominik Sala; Katarzyna Magiera-Mularz; Tad A. Holak

DOI: 10.3390/molecules25133017

Year of publication: 2020

Link: https://www.mdpi.com/1420-3049/25/13/3017

Di-bromo-Based Small-Molecule Inhibitors of the PD-1/PD-L1 Immune Checkpoint

Authors: Magdalena Konieczny; Bogdan Musielak; Justyna Kocik; Lukasz Skalniak; Dominik Sala; Mirosława Czub; Katarzyna Magiera-Mularz; Ismael Rodriguez; Maja Myrcha; Malgorzata Stec; Maciej Siedlar; Tad A. Holak; Jacek Plewka

DOI: 10.1021/acs.jmedchem.0c01260

Year of publication: 2020

Link: https://pubs.acs.org/doi/10.1021/acs.jmedchem.0c01260

Human and mouse PD-L1: similar molecular structure, but different druggability profiles

Authors: Katarzyna Magiera-Mularz; Justyna Kocik; Bogdan Musielak; Jacek Plewka; Dominik Sala; Monika Machula; Przemyslaw Grudnik; Malgorzata Hajduk; Marcin Czepiel; Maciej Siedlar; Tad A. Holak; Lukasz Skalniak

DOI: 10.1016/j.isci.2020.101960

Year of publication: 2020

Link: https://www.cell.com/iscience/fulltext/S2589-0042(20)31157-3?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2589004220311573%3Fshowall%3Dtrue

Macrocyclic Peptide Inhibitor of PD‐1/PD‐L1 Immune Checkpoint

Authors: Katarzyna Magiera-Mularz; Katarzyna Kuska; Lukasz Skalniak; Przemyslaw Grudnik; Bogdan Musielak; Jacek Plewka; Justyna Kocik; Malgorzata Stec; Kazimierz Weglarczyk; Dominik Sala; Benedykt Wladyka; Maciej Siedlar; Tad A. Holak; Grzegorz Dubin

DOI: 10.1002/adtp.202000195

Year of publication: 2020

Link: https://onlinelibrary.wiley.com/doi/10.1002/adtp.202000195

Multicomponent Peptide Stapling as a Diversity-Driven Tool for the Development of Inhibitors of Protein–Protein Interactions

Authors: Manuel G. Ricardo; Ameena M. Ali; Jacek Plewka; Ewa Surmiak; Beata Łabuzek; Constantinos G. Neochoritis; Jack Atmaj; Lukasz Skalniak; Ran Zhang; Tad A. Holak; Matthew Groves; Daniel G. Rivera; Alexander Dömling

DOI: 10.1002/anie.201916257

Year of publication: 2020

Link: https://onlinelibrary.wiley.com/doi/abs/10.1002/anie.201916257

Antibody Binding Heterogeneity of Protein A Resins

Authors: Gonçalo F. L. da Silva; Jacek Plewka; Rupert Tscheließnig; Helga Lichtenegger; Alois Jungbauer; Ana C. Dias-Cabral

DOI: 10.1002/biot.201800632

Year of publication: 2019

Link: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/biot.201800632

CA-170 – A Potent Small-Molecule PD-L1 Inhibitor or Not?

Authors: Bogdan Musielak; Justyna Kocik-Król; Łukasz Skalniak; Katarzyna Magiera-Mularz; Dominik Sala, Mirosława Czub, Małgorzata Stec, Maciej Siedlar, Tad A. Holak, Jacek Plewka

DOI: 10.3390/molecules24152804

Year of publication: 2019

Link: https://www.mdpi.com/1420-3049/24/15/2804

The pearl necklace model in protein A chromatography: Molecular mechanisms at the resin interface

Authors: Gonçalo L. Silva; Jacek Plewka; Helga C. Lichtenegger; Ana C. Dias-Cabral; Alois Jungbauer; Rupert Tscheließnig

DOI: 10.1002/bit.26843

Year of publication: 2019

Link: https://pubmed.ncbi.nlm.nih.gov/30252938/

Antibody adsorption in protein-A affinity chromatography-in-situ measurement of nanoscale structure by small-angle X-ray scattering

Authors: Jacek Plewka, Gonçalo L. Silva, Rupert Tscheließnig, Harald Rennhofer, Cristina Dias-Cabral, Alois Jungbauer, Helga C. Lichtenegger

DOI: 10.1002/jssc.201800776

Year of publication: 2018

Link: https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/10.1002/jssc.201800776

Viscoelasticity and structure of blood clots generated in-vitro by rheometry: A comparison between human, horse, rat, and camel

Authors: Christoph Dibiasi; Jacek Plewka; Leon Płoszczański; Veronika Glanz; Helga C. Lichtenegger; Ursula B. Windberger

DOI: 10.3233/CH-189203

Year of publication: 2018

Link: https://journals.sagepub.com/doi/10.3233/CH-189203

TRIM5α SPRY/coiled-coil interactions optimize avid retroviral capsid recognition

Authors: Marcin D. Roganowicz; Sevnur Komurlu; Santanu Mukherjee; Jacek Plewka; Steven L. Alam; Katarzyna A. Skorupka; Yueping Wan; Damian Dawidowski; David S. Cafiso; Barbie K. Ganser-Pornillos; Edward M. Campbell; Owen Pornillos

DOI: 10.1371/journal.ppat.1006686

Year of publication: 2017

Link: https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1006686